PXD038191 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Modelling organ-specific metastatic colonization of cholangiocarcinoma organoids in decellularized lung and lymph nodes |
Description | Cholangiocarcinoma (CCA) is a type of liver cancer with an aggressive phenotype and dismal outcome in patients. Current treatment options are limited, with surgical resection or transplantation the only possibility with curative intent. This becomes unavailable if CCA metastasizes to distant organs, most commonly lung and lymph nodes, which in turn drastically reduces overall survival. Clinically, these metastatic sites have a distinct impact on prognosis and survival, however mechanistic insight is lacking. This is partly due to currently available models that fail to mimic the complexity of tissue-specific environments for metastatic CCA. To create an in vitro model in which interaction between epithelial tumor cells and their surrounding extracellular matrix (ECM) can be studied in a metastatic setting, we combined patient-derived CCA organoids (CCAOs) (n=3) with decellularized human lung (n=3) and decellularized human lymph node (n=13). Decellularization resulted in removal of cells while preserving ECM structure and retaining important characteristics of the tissue origin. This includes a tissue-specific ECM protein signature and macro and micro-scale mechanical properties, which reveal the local heterogeneity of the ECM. After recellularization, gene expression analysis showed metastasis-related processes, including epithelial-to-mesenchymal transition, cancer stem cell plasticity, and ECM production, are significantly influenced by the ECM in an organ-specific manner. Furthermore, we find that CCAOs exhibit significant differences in migration and proliferation dynamics depending on metastatic site, the original patient tumor, and donor of target organ. Thus, CCA metastatic outgrowth is dictated both by the tumor itself as well as by the ECM of the target organ. In conclusion, convergence of CCAOs with its organ-specific location of metastasis, obtained by decellularization, provides a valuable tool for research on metastatic colonization in CCA. |
HostingRepository | PRIDE |
AnnounceDate | 2023-01-11 |
AnnouncementXML | Submission_2023-01-11_03:34:47.338.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | JeroenDemmers |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-11-17 03:53:40 | ID requested | |
⏵ 1 | 2023-01-11 03:34:47 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Metastatic colonization |
decellularization |
cholangiocarcinoma |
tumor organoids; |
Contact List
JeroenDemmers |
contact affiliation | Proteomics Center, Erasmus MC |
contact email | j.demmers@erasmusmc.nl |
lab head | |
JeroenDemmers |
contact affiliation | Proteomics Center, Erasmus University Medical Center, Rotterdam, The Netherlands |
contact email | j.demmers@erasmusmc.nl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD038191
- Label: PRIDE project
- Name: Modelling organ-specific metastatic colonization of cholangiocarcinoma organoids in decellularized lung and lymph nodes