PXD038132 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Chemoproteomic discovery of a human RNA ligase |
Description | RNA ligases are present across all forms of life. While enzymatic RNA ligation between 5'-PO4 and 3'-OH termini is prevalent in viruses, fungi, and plants, such RNA ligases are yet to be identified in vertebrates. Here, using a nucleotide-based chemical probe targeting human AMPylated proteome, we have enriched and identified the hitherto uncharacterised human protein chromosome 12 open reading frame 29 (C12orf29) as the first human enzyme promoting RNA ligation between 5'-PO4 and 3'-OH termini. C12orf29 catalyses ATP-dependent RNA ligation via a three-step mechanism, involving tandem auto- and RNA AMPylation. Knock-out of C12ORF29 gene impedes the cellular resilience to oxidative stress featuring concurrent RNA degradation, which suggests a role of C12orf29 in maintaining RNA integrity. This data provides the groundwork for establishing a human RNA repair pathway. |
HostingRepository | PRIDE |
AnnounceDate | 2023-01-27 |
AnnouncementXML | Submission_2023-01-26_23:56:56.782.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | PhilipSaumer |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; adenylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-11-14 02:02:50 | ID requested | |
⏵ 1 | 2023-01-26 23:56:57 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: RNA ligation, AMPylation,Proteomics, Post-translational modification |
Contact List
AndreasMarx |
contact affiliation | Organic Chemistry/Cellular Chemistry, Department of Chemistry, University of Konstanz |
contact email | andreas.marx@uni-konstanz.de |
lab head | |
PhilipSaumer |
contact affiliation | University of Konstanz |
contact email | philip.saumer@uni-konstanz.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD038132
- Label: PRIDE project
- Name: Chemoproteomic discovery of a human RNA ligase