PXD038027

PXD038027 is an original dataset announced via ProteomeXchange.

Title	Protocol for cell surface biotinylation of magnetic labeled and captured human peripheral blood mononuclear cells
Description	Analysis of the surfaceome of a blood cell subset requires cell sorting, followed by surface protein enrichment. Here, we present a protocol combining magnetically activated cell sorting (MACS) and surface biotinylation of the target cell subset from human peripheral blood mononuclear cells (PBMCs). We describe the steps for isolating target cells and their in-column surface biotinylation, followed by isolation and mass spectrometry analysis of biotinylated proteins. The protocol enables in-column surface biotinylation of specific cell subsets with minimal membrane disruption.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:59:20.891.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Servet ÖZCAN
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	biotinylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-07 10:19:59	ID requested
1	2022-11-08 01:56:27	announced
⏵ 2	2023-11-14 08:59:21	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: MACS, surface proteome, biotinylation

Servet ÖZCAN
contact affiliation	Faculty of Science, Department of Biology, Erciyes University, Kayseri 38039, Türkiye Genome and Stem Cell Center (GENKÖK), Erciyes University, Kayseri 38039, Türkiye
contact email	ozcan@erciyes.edu.tr
lab head
Servet ÖZCAN
contact affiliation	Erciyes University Genkök
contact email	ozcan@erciyes.edu.tr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/11/PXD038027

PRIDE project URI

• PRIDE
  1. PXD038027
     1. Label: PRIDE project
     2. Name: Protocol for cell surface biotinylation of magnetic labeled and captured human peripheral blood mononuclear cells