PXD037971

PXD037971 is an original dataset announced via ProteomeXchange.

Title	Subcellular Localization Shapes the Fate of RNA Polymerase III
Description	RNA Pol III plays a vital role in transcription and as a viral-DNA sensor, but how it is assembled and distributed within cells remain poorly understood. Here, we show that Pol III is assembled with chaperones in the cytoplasm and forms transcription-dependent protein clusters upon transport into the nucleus. The largest subunit (RPC1) depletion through an auxin-inducible degron leads to rapid degradation and disassembly of Pol III complex in the nucleus and cytoplasm, respectively. This generates a pool of partially assembled Pol III intermediates, which can be rapidly mobilized into the nucleus upon the restoration of RPC1. Our study highlights the critical role of subcellular localization in determining Pol III's fate and provide insight into the dynamic regulation of nuclear Pol III levels and the origin of cytoplasmic Pol III complexes involved in mediating viral immunity.
HostingRepository	iProX
AnnounceDate	2022-11-06
AnnouncementXML	Submission_2023-08-22_19:51:23.562.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kai Tian
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-07 00:06:38	ID requested
⏵ 1	2023-08-22 19:51:23	announced

Dataset with its publication pending

submitter keyword: turboID, mESC, pol III, subunit degradation, subcellular fraction

Xiong Ji
contact affiliation	Peking University
contact email	xiongji@pku.edu.cn
lab head
Kai Tian
contact affiliation	Peking university
contact email	tiankai_ioz@163.com
dataset submitter

iProX dataset URI