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PXD037859

PXD037859 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSynapse integrity and function: dependence on protein synthesis and identification of potential failure points
DescriptionSynaptic integrity and function depend on myriad proteins - labile molecules with finite lifetimes that need to be continually replaced with freshly synthesized copies. Here we describe experiments designed to expose synaptic (and neuronal) properties and functions that are particularly sensitive to disruptions in protein supply, identify proteins lost early upon such disruptions, and uncover potential, yet currently underappreciated failure points. We report here that acute suppressions of protein synthesis are followed within hours by reductions in spontaneous network activity levels, impaired oxidative phosphorylation and mitochondrial function, reduced tenacity of both excitatory and inhibitory synapses and loss of synapses of both classes. Conversely, gross impairments in presynaptic vesicle recycling are observed only after several days, as is overt cell death. Proteomic analysis identified groups of potentially essential ‘early-lost’ proteins including regulators of synapse stability, proteins related to bioenergetics, fatty acid and lipid metabolism, and, unexpectedly, numerous proteins involved in Alzheimer’s disease pathology and amyloid beta processing.
HostingRepositoryPRIDE
AnnounceDate2022-11-27
AnnouncementXMLSubmission_2022-11-27_00:42:23.370.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterTamarZiv
SpeciesList scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-10-31 09:21:58ID requested
12022-11-27 00:42:23announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: SiLAC, protein synthesis,Synaptic integrity
Contact List
Professor Noam E.Ziv
contact affiliationRappaport Faculty of Medicine and Network Biology Research Laboratories Fishbach Building, room 432 Technion City, Haifa 32000 Israel
contact emailnoamz@technion.ac.il
lab head
TamarZiv
contact affiliationTechnion
contact emailtamarz@technion.ac.il
dataset submitter
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Dataset FTP location
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