PXD037736

PXD037736 is an original dataset announced via ProteomeXchange.

Title	Coupled induction of prophage and virulence factors during tick transmission of the Lyme disease spirochete
Description	The alternative sigma factor RpoS plays a central role in the critical host-adaptive response of the Lyme disease spirochete, Borrelia burgdorferi. We previously identified bbd18 as a negative regulator of RpoS but could not inactivate bbd18 in wild-type spirochetes. In the current study we employed an inducible bbd18 gene to demonstrate the essential nature of BBD18 for viability of wild-type spirochete viability in vitro and at a unique point in vivo. Transcriptomic analyses of BBD18 depleted cells demonstrated global induction of RpoS-dependent genes prior to lysis, with the absolute requirement for BBD18, both in vitro and in vivo, circumvented by deletion of rpoS. The increased expression of plasmid prophage genes and the presence of phage particles in the supernatants of lysing cultures indicate that RpoS regulates phage lysis-lysogeny decisions. Through this work we identify a mechanistic link between endogenous transducing prophages and the RpoS-dependent adaptive response of the Lyme disease spirochete. The alternative sigma factor RpoS plays a central role in the critical host-adaptive response of the Lyme disease spirochete, Borrelia burgdorferi. We previously identified bbd18 as a negative regulator of RpoS but could not inactivate bbd18 in wild-type spirochetes. In the current study we employed an inducible bbd18 gene to demonstrate the essential nature of BBD18 for viability of wild-type spirochete viability in vitro and at a unique point in vivo. Transcriptomic analyses of BBD18 depleted cells demonstrated global induction of RpoS-dependent genes prior to lysis, with the absolute requirement for BBD18, both in vitro and in vivo, circumvented by deletion of rpoS. The increased expression of plasmid prophage genes and the presence of phage particles in the supernatants of lysing cultures indicate that RpoS regulates phage lysis-lysogeny decisions. Through this work we identify a mechanistic link between endogenous transducing prophages and the RpoS-dependent adaptive response of the Lyme disease spirochete.
HostingRepository	PRIDE
AnnounceDate	2022-12-12
AnnouncementXML	Submission_2022-12-12_09:22:33.131.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	JennyWachter
SpeciesList	scientific name: Borrelia burgdorferi B31; NCBI TaxID: 224326;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-26 01:33:27	ID requested
⏵ 1	2022-12-12 09:22:33	announced

Dataset with its publication pending

submitter keyword: Borrelia burgdorferi, bbd18, rpoS

PatriciaRosa
contact affiliation	Molecular Genetics Section Laboratory of Bacteriology Rocky Mountain Laboratories, DIR, NIAID, NIH USA
contact email	prosa@niaid.nih.gov
lab head
JennyWachter
contact affiliation	Vaccine and Infectious Disease Organization
contact email	jenny.wachter@usask.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD037736
     1. Label: PRIDE project
     2. Name: Coupled induction of prophage and virulence factors during tick transmission of the Lyme disease spirochete