PXD037611
PXD037611 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Ammonium Sulfate-based Prefractionation Improved Carbonylated Proteome Coverage in Arabidopsis thaliana Leaf Extract |
Description | The hypothesis that ammonium sulfate-based protein precipitation can help prefractionate the total protein extract into fractions of reduced complexity and improve the detection of low-abundance proteins was investigated. This dataset resulted from the experiments that we performed to improve the detection of the carbonylated proteins in leaf extract. A total of 122 carbonylated proteins were identified, of which 77 were found in the ammonium sulfate fractions only. This prefractionation method allowed to identify 63% more carbonylated proteins compared to the number of carbonylated proteins identified from the total crude extract without prefractionation. Our results thus showed that a simple offline prefractionation of the proteome by ammonium sulfate prior to liquid chromatography-tandem mass spectrometry significantly improved the detection of carbonylated proteins in Arabidopsis leaf extract. |
HostingRepository | MassIVE |
AnnounceDate | 2023-02-19 |
AnnouncementXML | Submission_2023-02-19_10:44:13.059.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Tagnon Missihoun |
SpeciesList | scientific name: Arabidopsis thaliana; common name: thale cress; NCBI TaxID: 3702; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2022-10-20 11:25:25 | ID requested | |
⏵ 1 | 2023-02-19 10:44:13 | announced |
Publication List
no publication |
Keyword List
submitter keyword: Aldehyde reactive probe, peptide level enrichment, protein carbonylation, proteome offline prefractionation |
Contact List
Tagnon Missihoun | |
---|---|
contact affiliation | University of Quebec Trois-Rivieres |
contact email | Tagnon.Missihoun@uqtr.ca |
lab head | |
Tagnon Missihoun | |
contact affiliation | University of Quebec Trois-Rivieres |
contact email | Tagnon.Missihoun@uqtr.ca |
dataset submitter |
Full Dataset Link List
MassIVE dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000090559/ |