PXD037490 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Synaptic vesicle proteins and ATG9A self-organize in distinct vesicle phases within synapsin condensates |
Description | Ectopic expression in fibroblasts of synapsin 1 and synaptophysin is sufficient to generate condensates of vesicles highly reminiscent of synaptic vesicle (SV) clusters and with liquid-like properties. We show that unlike synaptophysin, other major integral SV membrane proteins fail to form condensates with synapsin, but coassemble into the clusters formed by synaptophysin and synapsin in this ectopic expression system. Another vesicle membrane protein, ATG9A, undergoes activity-dependent exo-endocytosis at synapses, raising questions about the relation of ATG9A traffic to the traffic of SVs. We have found that both in fibroblasts and in nerve terminals ATG9A does not coassemble into synaptophysin-positive vesicle condensates but localizes on a distinct class of vesicles that also assembles with synapsin but into a distinct phase. Our findings suggest that ATG9A undergoes differential sorting relative to SV proteins and also point to a dual role of synapsin in controlling clustering at synapses of SVs and ATG9A vesicles |
HostingRepository | PRIDE |
AnnounceDate | 2022-12-24 |
AnnouncementXML | Submission_2022-12-23_19:44:37.086.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Zon WengLai |
SpeciesList | scientific name: Cercopithecus aethiops (Green monkey) (Grivet); NCBI TaxID: 9534; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-10-17 01:28:18 | ID requested | |
⏵ 1 | 2022-12-23 19:44:37 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: synaptophysin, synapsin, ATG9A, sypnatic vesicle |
Contact List
Pietro DeCamilli |
contact affiliation | Yale University School of Medicine |
contact email | pietro.decamilli@yale.edu |
lab head | |
Zon WengLai |
contact affiliation | Harvard T. H. Chan School of Public Health |
contact email | zlai@hsph.harvard.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD037490
- Label: PRIDE project
- Name: Synaptic vesicle proteins and ATG9A self-organize in distinct vesicle phases within synapsin condensates