PXD037464 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Dynamic partitioning of branched-chain amino acids- derived nitrogen supports renal cancer progression |
Description | Metabolic reprogramming is critical for tumor initiation and progression. However, the exact impact of specific metabolic changes on cancer progression is poorly understood. Here, we integrate multimodal analyses of primary and metastatic clonally-related clear cell renal cancer cells (ccRCC) grown in physiological media to identify key stage-specific metabolic vulnerabilities. We show that a VHL loss-dependent reprogramming of branched-chain amino acid catabolism sustains the de novo biosynthesis of aspartate and arginine enabling tumor cells with the flexibility of partitioning the nitrogen of the amino acids depending on their needs. Importantly, we identify the epigenetic reactivation of argininosuccinate synthase (ASS1), a urea cycle enzyme suppressed in primary ccRCC, as a crucial event for metastatic renal cancer cells to acquire the capability to generate arginine, invade in vitro and metastasize in vivo. Overall, our study uncovers a mechanism of metabolic flexibility occurring during ccRCC progression, paving the way for the development of novel stage-specific therapies. |
HostingRepository | PRIDE |
AnnounceDate | 2022-11-09 |
AnnouncementXML | Submission_2022-11-09_07:26:45.805.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Alexvon kriegsheim |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-10-15 14:38:34 | ID requested | |
⏵ 1 | 2022-11-09 07:26:46 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Metabolism, Renal Cancer |
Contact List
Alex vonKriegsheim |
contact affiliation | University of Edinburgh |
contact email | avonkri@ed.ac.uk |
lab head | |
Alexvon kriegsheim |
contact affiliation | University of Edinburgh |
contact email | alex.vonkriegsheim@ucd.ie |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD037464
- Label: PRIDE project
- Name: Dynamic partitioning of branched-chain amino acids- derived nitrogen supports renal cancer progression