PXD037402

PXD037402 is an original dataset announced via ProteomeXchange.

Title	Chemical proteomics reveals protein tyrosination extends beyond the alpha-tubulins in human cells.
Description	Tubulin detyrosination-tyrosination cycle regulates the stability of microtubules. Thus far described on α-tubulins, the tyrosination level is maintained by a single tubulin-tyrosine ligase (TTL). However, the precise dynamics and tubulin isoforms which undergo (de)tyrosination in neurons are unknown. Here, we exploit the substrate promiscuity of the TTL to introduce an O-propargyl-L-tyrosine in cancer cell lines and neurons. Mass spectrometry-based chemical proteomics in neuroblastoma cells using the O-propargyl-L-tyrosine probe revealed previously discussed tyrosination of TUBA4A, MAPRE1, and other non-tubulin proteins. This finding was further corroborated in differentiating neurons. We present the method for tubulin tyrosination profiling in living cells. Our results show that detyrosination-tyrosination is not restricted to α-tubulins with coded C-terminal tyrosine and is thus involved in fine-tuning of the tubulin and non-tubulin proteins during neuronal differentiation.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:59:31.850.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dmytro Makarov
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-13 15:34:10	ID requested
1	2022-10-20 01:22:35	announced
⏵ 2	2023-11-14 08:59:32	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: iNGNs, DIA-NN,Tyrosination, SP2E, SH-SY5Y

Dr. Pavel Kielkowski
contact affiliation	LMU München, Department of Chemistry. Institute for Chemical Epigenetics – Munich (ICEM)
contact email	pavel.kielkowski@cup.lmu.de
lab head
Dmytro Makarov
contact affiliation	PhD
contact email	d.makarov@lmu.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD037402
     1. Label: PRIDE project
     2. Name: Chemical proteomics reveals protein tyrosination extends beyond the alpha-tubulins in human cells.