PXD037350
PXD037350 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Multi-Comparative Thermal Proteome Profiling Uncovers New O-GlcNAc Proteins in System-Wide |
Description | Among diverse protein post-translational modifications (PTMs), O-GlcNAcylation, a simple but essential monosaccharide modification, plays crucial roles in cellular processes and is closely related to various diseases. Despite its ubiquity in cells, the properties of low stoichiometry and reversibility are hard nuts to crack in system-wide research of O-GlcNAc. Herein, we developed a novel method employing multi-comparative thermal proteome profiling for OGT substrates discovery. Melting curves of proteins under different treatments were profiled and compared with high reproducibility and consistency. Conse-quently, proteins with significant shifted stabilities caused by OGT and UDP-GlcNAc were screened out, from which new O-GlcNAcylated proteins were uncovered. |
HostingRepository | iProX |
AnnounceDate | 2022-10-13 |
AnnouncementXML | Submission_2022-12-06_18:21:31.532.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Guoli Wang |
SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2022-10-12 22:51:46 | ID requested | |
⏵ 1 | 2022-12-06 18:21:31 | announced |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: O-GlcNAcyaltion |
Contact List
Haojie Lu | |
---|---|
contact affiliation | Fudan University |
contact email | luhaojie@fudan.edu.cn |
lab head | |
Guoli Wang | |
contact affiliation | Fudan University |
contact email | 19111510006@fudan.edu.cn |
dataset submitter |
Full Dataset Link List
iProX dataset URI |