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PXD037230

PXD037230 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleScleraxis-lineage cells are required for tendon homeostasis and their depletion induces an accelerated extracellular matrix aging phenotype
DescriptionAged tendons have disrupted homeostasis, increased injury risk, and impaired healing capacity. Understanding mechanisms of homeostatic disruption is crucial for developing therapeutics to retain tendon health through the lifespan. Here, we developed a novel model of accelerated tendon extracellular matrix (ECM) aging via depletion of Scleraxis-lineage (ScxLin) cells in young mice (DTR). DTR recapitulates many aspects of tendon aging including comparable declines in cellularity, alterations in ECM structure, organization, and composition. Single cell RNA-sequencing demonstrated a conserved decline in tenocytes associated with ECM biosynthesis in aged and DTR tendons, identifying the requirement for ScxLin cells during homeostasis. However, the remaining cells in aged and DTR tendons demonstrate functional divergence. Aged tenocytes become pro-inflammatory and lose proteostasis. In contrast, DTR tenocytes demonstrate enhanced remodeling capacity. Collectively, this study defines DTR a novel model of accelerated tendon ECM aging and identifies novel biological intervention points to maintain tendon function through the lifespan.
HostingRepositoryPRIDE
AnnounceDate2023-02-03
AnnouncementXMLSubmission_2023-02-03_08:14:08.951.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterKyleSwovick
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-10-10 07:06:43ID requested
12023-02-03 08:14:09announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Extracellular Matrix, Tendon,Aging, Mouse, Scleraxis, LC-MS/MS
Contact List
Alayna E.Loiselle
contact affiliationCenter for Musculoskeletal Research, Department of Orthopaedics & Rehabilitation, University of Rochester Medical Center, Rochester, NY, 14642 Department of Biomedical Engineering, University of Rochester, Rochester, NY, 14627
contact emailAlayna_Loiselle@URMC.Rochester.edu
lab head
KyleSwovick
contact affiliationUniversity of Rochester Medical Center Mass Spectrometry Shared Resource Laboratory
contact emailkswovick@ur.rochester.edu
dataset submitter
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Dataset FTP location
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