PXD037230 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Scleraxis-lineage cells are required for tendon homeostasis and their depletion induces an accelerated extracellular matrix aging phenotype |
Description | Aged tendons have disrupted homeostasis, increased injury risk, and impaired healing capacity. Understanding mechanisms of homeostatic disruption is crucial for developing therapeutics to retain tendon health through the lifespan. Here, we developed a novel model of accelerated tendon extracellular matrix (ECM) aging via depletion of Scleraxis-lineage (ScxLin) cells in young mice (DTR). DTR recapitulates many aspects of tendon aging including comparable declines in cellularity, alterations in ECM structure, organization, and composition. Single cell RNA-sequencing demonstrated a conserved decline in tenocytes associated with ECM biosynthesis in aged and DTR tendons, identifying the requirement for ScxLin cells during homeostasis. However, the remaining cells in aged and DTR tendons demonstrate functional divergence. Aged tenocytes become pro-inflammatory and lose proteostasis. In contrast, DTR tenocytes demonstrate enhanced remodeling capacity. Collectively, this study defines DTR a novel model of accelerated tendon ECM aging and identifies novel biological intervention points to maintain tendon function through the lifespan. |
HostingRepository | PRIDE |
AnnounceDate | 2023-02-03 |
AnnouncementXML | Submission_2023-02-03_08:14:08.951.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | KyleSwovick |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-10-10 07:06:43 | ID requested | |
⏵ 1 | 2023-02-03 08:14:09 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Extracellular Matrix, Tendon,Aging, Mouse, Scleraxis, LC-MS/MS |
Contact List
Alayna E.Loiselle |
contact affiliation | Center for Musculoskeletal Research, Department of Orthopaedics & Rehabilitation, University of Rochester Medical Center, Rochester, NY, 14642 Department of Biomedical Engineering, University of Rochester, Rochester, NY, 14627 |
contact email | Alayna_Loiselle@URMC.Rochester.edu |
lab head | |
KyleSwovick |
contact affiliation | University of Rochester Medical Center Mass Spectrometry Shared Resource Laboratory |
contact email | kswovick@ur.rochester.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/02/PXD037230 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD037230
- Label: PRIDE project
- Name: Scleraxis-lineage cells are required for tendon homeostasis and their depletion induces an accelerated extracellular matrix aging phenotype