PXD037040

PXD037040 is an original dataset announced via ProteomeXchange.

Title	Tryptase regulation of joint lubrication and inflammation in osteoarthritis
Description	Proteoglycan 4 (PRG4) is an extracellular matrix protein that maintains homeostasis through its boundary lubricating and anti-inflammatory properties. Altered expression and function of PRG4 have been associated with joint inflammatory diseases, including osteoarthritis (OA). We found that mast cell tryptase cleaves PRG4 in a dose- and time-dependent manner, which was confirmed by silver stain gel electrophoresis and mass spectrometry. Tryptase-treated PRG4 resulted in a reduction of lubrication. Compared to full-length, processed PRG4 was shown to further activate NF-B expression in cells overexpressing TLR2, -4, and -5. In the destabilization of the medial meniscus (DMM) model of OA in rat, tryptase and PRG4 colocalized at the site of injury in knee cartilage and were associated with disease severity. Primary synovial fibroblasts from OA patients or healthy subjects treated with tryptase and/or PRG4, and synovial cells from OA patients, were subjected to a quantitative shotgun proteomics and proteome changes were characterized, further supporting the role of NF-B activation. We identified tryptase as a modulator of joint lubrication via the processing of PRG4 in OA.
HostingRepository	PRIDE
AnnounceDate	2023-03-04
AnnouncementXML	Submission_2023-03-04_07:35:08.775.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	NabangshuDas
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; acetylated residue; monohydroxylated residue; deamidated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-27 11:35:43	ID requested
⏵ 1	2023-03-04 07:35:09	announced

Dataset with its publication pending

submitter keyword: Proteoglycan-4,Tryptase, Proteases, Single-cell RNA-seq, Lubricin, Osteoarthritis, Lubrication, Proteomics, N-terminomics

AntoineDufour
contact affiliation	McCaig institute for Bone and Joint Health, Cumming School of Medicine, University of Calgary, AB, Canada
contact email	antoine.dufour@ucalgary.ca
lab head
NabangshuDas
contact affiliation	University of Calgay
contact email	nabangshu.das@ucalgary.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD037040
     1. Label: PRIDE project
     2. Name: Tryptase regulation of joint lubrication and inflammation in osteoarthritis