PXD036954

PXD036954 is an original dataset announced via ProteomeXchange.

Title	Identification of novel kinases of Tau using fluorescence complementation mass spectrometry (FCMS)
Description	Hyperphosphorylation of the microtubule-associated protein Tau is a major hallmark of Alzheimer’s disease (AD) and other tauopathies. Understanding the protein kinases that phosphorylate Tau is critical for the development of new drugs that target Tau phosphorylation. At present, the repertoire of the Tau kinases remains incomplete, and methods to uncover novel upstream protein kinases are still limited. Here, we apply our newly developed proteomic strategy, fluorescence complementation mass spectrometry (FCMS), to identify novel kinase candidates of Tau. By constructing Tau- and kinase-fluorescent fragment library, we detected 59 Tau-associated kinases, including 23 known kinases of Tau and 36 novel candidate kinases. In the validation phase using in vitro phosphorylation, four kinase candidates, OXSR1, DAPK2, CSK, and ZAP70, displayed the ability to phosphorylate Tau. Furthermore, co-expression of these four kinases along with Tau increased the phosphorylation of Tau in human neuroglioma H4 cells. Taken together, we demonstrate the proteomic strategy to systematically identify potential kinases that can phosphorylate Tau in cells. Our discovery of new candidate kinases of Tau can present new opportunities for developing AD therapeutic strategies.
HostingRepository	jPOST
AnnounceDate	2023-09-23
AnnouncementXML	Submission_2023-09-22_08:00:06.542.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Der-Shyang Kao
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine; TMT6plex reporter+balance reagent N-acylated residue; TMT6plex reporter+balance reagent acylated N-terminal
Instrument	instrument; instrument; instrument

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-22 22:30:53	ID requested
⏵ 1	2023-09-22 08:00:06	announced

Dataset with its publication pending

submitter keyword: kinases, Tau

W. Andy Tao
lab head
Der-Shyang Kao
contact affiliation	Purdue University
dataset submitter

jPOST dataset URI

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