PXD036953 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The interactome of flavin adenine dinucleotide (FAD) with RNA-binding proteins |
Description | We explored the interactome of flavin adenine dinucleotide (FAD) and the matrix of UV-immobilised FAD bound more than 3,000 proteins from SW-620 lysate. GO enrichment analysis showed that about 600 RNA-binding proteins were bound to FAD beads and, surprisingly, more than 40 proteins showed clear dose-dependent binding competition with nanomolar affinities indicating that their direct or indirect interaction with free FAD is both specific and strong. Among the few known consensus sequences for RNA-binding proteins, we selected the PUF motif 5'-UGUANAUA-3' to investigate whether FAD is binding the Pumilio homologs PUM1 and PUM2 in their RNA-binding pocket, as both these proteins showed dose-response behaviour in the FAD versus FAD-beads. Immobilisation of an oligomer containing the consensus sequence on NHS-activated Sepharose beads yielded PUM-beads that did enrich the Pumilio homologs. Oligomer vs FAD-beads and FAD vs PUM-beads together with the FAD vs FAD-beads dose-response competition indicated that the binding of FAD does not seem to influence the binding of the RNA. It is therefore most likely that FAD binding is allosteric for PUMs. |
HostingRepository | PRIDE |
AnnounceDate | 2022-10-06 |
AnnouncementXML | Submission_2022-10-06_04:17:25.997.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Polina Prokofeva |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-09-22 05:53:00 | ID requested | |
⏵ 1 | 2022-10-06 04:17:26 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: PUM-RNA, FAD, competition pulldown, photo-immobilisation |
Contact List
Bernhard Kuster |
contact affiliation | Chair of Proteomics and Bioanalytics, TUM School of Life Sciences, Technical University of Munich, 85354 Freising, Germany |
contact email | kuster@tum.de |
lab head | |
Polina Prokofeva |
contact affiliation | Chair of Proteomics and Bioanalytics, Technical University of Munich, Emil-Erlenmeyer-Forum 5, 85354 Freising, Germany |
contact email | polina.prokofeva@tum.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD036953
- Label: PRIDE project
- Name: The interactome of flavin adenine dinucleotide (FAD) with RNA-binding proteins