PXD036520

PXD036520 is an original dataset announced via ProteomeXchange.

Title	Proteomic profiling of tissue-engineered, small-diameter vascular grafts implanted into the ovine carotid artery
Description	Albeit vascular prostheses for the replacement of large arteries (e.g. aorta) are commercially available for decades, small-diameter vascular grafts (e.g., for coronary artery bypass graft surgery) still remain an unmet clinical need. Biostable polymers commonly used for the fabrication of aortic prostheses (e.g., poly(ethylene terephthalate) or expanded poly(tetrafluoroethylene)) have insufficient haemocompatibility to withstand thrombosis at low blood flow characteristic of small arteries (e.g., coronary artery). Hence, researchers endeavor to develop a biodegradable, tissue-engineered vascular graft (TEVG) to avoid the use of autologous blood vessels, such as saphenous vein or internal mammary artery, as conduits during the bypass surgery. Although a number of promising prototypes have been designed to date, none of them passed the pre-clinical trials successfully. Implantation into the ovine or porcine arteries is associated with thrombosis, neointimal hyperplasia, and aneurysms within one-year postoperation, precluding further clinical translation of TEVGs. Among the reasons of such impediment is that pathophysiology of TEVG implantation remains unclear and the molecular events occurring in the TEVG upon its implantation have not been properly investigated hitherto. Here, we for the first time performed a proteomic profiling of the TEVGs (n = 12) implanted into the ovine carotid arteries for one year and suffered from thrombosis to identify the signatures of TEVG failure in an unbiased manner. Contralateral intact ovine carotid arteries (n = 12) have been selected as a control group.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:58:20.088.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Arseniy Lobov
SpeciesList	scientific name: Ovis aries; NCBI TaxID: 9940;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-05 09:37:16	ID requested
1	2022-11-26 04:51:50	announced
⏵ 2	2023-11-14 08:58:20	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: shotgun proteomics, TimsToF Pro, vascular grafts, Ovis aries,sheep

Anton G. Kutikhin
contact affiliation	Head of the Laboratory for Vascular Biology, Department of Experimental Medicine,Research Institute for Complex Issues of Cardiovascular Diseases, Kemerovo, Russian Federation
contact email	antonkutikhin@gmail.com
lab head
Arseniy Lobov
contact affiliation	Emmanuel Levy Lab, Dept. of Chemical and Structural Biology, Weizmann Institute of Science
contact email	arseniylobov@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD036520
     1. Label: PRIDE project
     2. Name: Proteomic profiling of tissue-engineered, small-diameter vascular grafts implanted into the ovine carotid artery