PXD036511 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | ER-Golgi membrane contact sites control the formation of plasma membrane lipid nanodomains required for anthrax intoxication |
Description | To promote infections, pathogens exploit host cell machineries including structural elements of the plasma membrane. Studying these interactions and identifying molecular players is an ideal way to gain insights into the fundamental biology of the host cell. Here, we used the anthrax toxin to screen a library of 1500 regulatory, cell surface, and membrane trafficking genes for their involvement in the intoxication process. We found that ER-Golgi localized proteins TMED2 and TMED10 are required for toxin oligomerization at the plasma-membrane of human cells, an essential step dependant on localization to cholesterol-rich lipid nanodomains. Biochemical, morphological and mechanistic analyses showed that TMED2 and TMED10 are essential components of a complex that operates the exchange of both cholesterol and ceramides at ER-Golgi membrane contact sites. Overall, this study of anthrax intoxication led to the discovery that lipid compositional remodelling at ER-Golgi interfaces fully controls the formation of functional membrane nanodomains at the cell surface. |
HostingRepository | PRIDE |
AnnounceDate | 2022-09-07 |
AnnouncementXML | Submission_2022-09-07_12:04:06.050.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Muhammad Umair Anwar |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-09-05 09:09:20 | ID requested | |
⏵ 1 | 2022-09-07 12:04:06 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: p24 family, TMED2, TMED10, nanodomains, anthrax toxin, membrane contact sites, OSBP, VAPA |
Contact List
F. Gisou van der Goot |
contact affiliation | Global Health Institute, School of Life Sciences, EPFL, 1015 Lausanne, Switzerland |
contact email | gisou.vandergoot@epfl.ch |
lab head | |
Muhammad Umair Anwar |
contact affiliation | École Polytechnique Fédérale de Lausanne |
contact email | muhammad.anwar@epfl.ch |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/09/PXD036511 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD036511
- Label: PRIDE project
- Name: ER-Golgi membrane contact sites control the formation of plasma membrane lipid nanodomains required for anthrax intoxication