PXD036406

PXD036406 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Multistate structures of the MLL1-WRAD complex bound to H2B-ubiquitinated nucleosome
Description	The human Mixed Lineage Leukemia-1 (MLL1) complex orchestrates methylation of histone H3K4 to promote transcription and is the target of chromosomal translocations in a variety of leukemias. The MLL1 core complex comprises the MLL1 methyltransferase and the WRAD complex, which contains WDR5, RbBp5, Ash2L, and DPY-30. Recent studies have shed light on the structure and organization of the human MLL1-WRAD complex bound to nucleosomes but were not able to resolve portions of the Ash2L subunit or DPY30. We used an integrated approach combining cryo-electron microscopy and mass spectrometry-cross linking to obtain models of the MLL1-WRAD complex bound to nucleosomes containing monoubiquitinated histone H2B, which is reported to stimulate H3K4 methylation. We constructed a model containing the Ash2L intrinsically disordered region (IDR), SPRY insertion region, and Sdc1-DPY30 interacting region (SDI-motif), as well as the DPY30 dimer, which were not resolved in previous structures. In addition to the model of the active state, we resolved three additional states of MLL1-WRAD lacking one or more subunits that may reflect different steps in the assembly of MLL1-WRAD. Our results provide a more complete picture of MLL1-WRAD and how it assembles on nucleosomes to form an active methyltransferase complex.
HostingRepository	jPOST
AnnounceDate	2023-08-31
AnnouncementXML	Submission_2023-08-30_08:00:05.079.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bruce Knutson
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide
Instrument	LTQ Orbitrap Elite

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-30 13:00:13	ID requested
⏵ 1	2023-08-30 08:00:05	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: MLL1, WRAD, H2B, Ubiquitin, CXMS, XLMS, crosslinking, BS3

submitter keyword: MLL1, WRAD, H2B, Ubiquitin, CXMS, XLMS, crosslinking, BS3

Contact List

Bruce A Knutson
lab head
Bruce Knutson
contact affiliation	SUNY Upstate Medical University
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001677/