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PXD036093

PXD036093 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomics Reveals the Potential Mechanism of Tanshinone IIA Promoting the Ex Vivo Expansion of Human Bone Marrow Mesenchymal Stem Cells.
DescriptionBone marrow mesenchymal stem cells (BMSCs) is a promising cell type for tissue engineering, however, the application of BMSCs are largely hampered for the limited number harvested from bone marrow cells. The methods or strategies that focused on promoting the capacity of BMSCs expansion ex vivo become more and more important. Tanshinone IIA (Tan IIA), the main active components of Danshen, has been found to promote BMSCs proliferation, but the underlying mechanism is still unclear. This present work employed biological techniques combined with quantitative proteome analysis to explore the effect and underlying mechanism of Tan IIA on the ex vivo expansion capacity of BMSCs from human. The results showed that a total of 84 differentially expressed proteins were annotated and among them 51 upregulated proteins and 33 downregulated proteins were identified. Besides, Tan IIA could promote hBMSCs proliferation by regulating the progression of S phase via increasing the release of fibroblast growth factor 2 (FGF2), FGF-mediated PI3K/AKT signaling pathways may play an important role in Tan IIA’s effect on hBMSCs expansion. The results of this study may provide further evidence about the potential of Tan IIA to be used for the research of hBMSCs therapies.
HostingRepositoryPRIDE
AnnounceDate2022-11-28
AnnouncementXMLSubmission_2022-11-28_07:50:05.772.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterPeiYuan
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-08-16 13:31:10ID requested
12022-11-28 07:50:06announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: bone marrow mesenchymal stem cells
Tanshinone IIA
proteomics
expansion
fibroblast growth factor 2
autocrine.
Contact List
XunLi
contact affiliationThe First Clinical Medical College, Lanzhou University; Key Laboratory of Biotherapy and Regenerative Medicine of Gansu Province; The Medical Frontier Innovation Research Center, The First Hospital of Lanzhou University; The Department of General Surgery, The First Hospital of Lanzhou University; Hepatopancreatobiliary Surgery Institute of Gansu Province, Lanzhou 730000, Gansu Province, China.
contact emailyuanp19@lzu.edu.cn
lab head
PeiYuan
contact affiliationThe First Clinical Medical College of Lanzhou University
contact emailyuanp19@lzu.edu.cn
dataset submitter
Full Dataset Link List
Dataset FTP location
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