PXD035818

PXD035818 is an original dataset announced via ProteomeXchange.

Title	Characterization of Phosphorylation-dependent Antibody Binding to Cancer-mutated Linkers of C2H2 Zinc Finger Proteins by Intact Transition Epitope Mapping - Thermodynamic Weak-force Order Analysis.
Description	We describe mass spectrometric antibody-peptide binding motif analyses of a monoclonal antibody (clone 18E9.D9, BioLegend, London, England) from mouse which was raised against the predominantly occuring phosphorylated linker sequence HpTGEKP of human C2H2 zinc finger proteins. As the anti-HpTGEKP antibody is assumed to bind to related phosphorylated C2H2 ZNF protein linker sequences as well, specific amino acid exchanges were investigated for evaluating their importance for immune complex formation. Two point mutations of the zinc finger protein 331 (ZNF331) gene, causing single amino acid exchanges R9I and H11Y, respectively, have been found to be significantly enriched in Uterine Corpus Endometrial Carcinoma, Colon and Rectal Adenocarcinomas, and Skin Cutaneous Melanoma. Since the H11Y amino acid exchange affects histidine residue H1 of the HpTGEKP linker motif which is recognized by the anti-HpTGEKP antibody, we examined in this work whether the in several cancers mutated linker amino acid sequence YTGEKP and its phosphorylated derivatives YpTGEKP, pYTGEKP, and pYpTGEKP would be bound by the anti-HpTGEKP antibody. Immune complex formation ability was compared to that of peptides HTGEKP, HpTGEKP, and HpTGKKP, respectively, which served as controls. Binding behaviors towards the anti-HpTGEKP antibody were investigated by "Intact Transmission Epitope Mapping - Thermodynamic Weak-force Order (ITEM-TWO)".
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:59:50.801.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michael Kreutzer
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-04 08:18:42	ID requested
1	2023-01-24 22:30:46	announced
⏵ 2	2023-11-14 08:59:51	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: ITEM-TWO,Off-line ESI-MS, Uterine Corpus Endometrial Carcinoma, Skin Cutaneous Melanoma, zinc finger-phosphopeptide, antiPhosphopeptide-antibody, Colon and Rectal Adenocarcinomas

Prof. Dr. Michael O. Glocker
contact affiliation	Director, Proteome Center Rostock, University of Rostock, Germany
contact email	michael.glocker@med.uni-rostock.de
lab head
Michael Kreutzer
contact affiliation	University Medical Center Rostock
contact email	michael.kreutzer@med.uni-rostock.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD035818
     1. Label: PRIDE project
     2. Name: Characterization of Phosphorylation-dependent Antibody Binding to Cancer-mutated Linkers of C2H2 Zinc Finger Proteins by Intact Transition Epitope Mapping - Thermodynamic Weak-force Order Analysis.