PXD035761 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identification of putative reader proteins of RNA 5-methylcytosine and its derivatives in Caenorhabditis elegans |
Description | Methylation of carbon-5 of cytosines (m5C) is a conserved post-transcriptional nucleotide modification of RNA with widespread distribution across organisms. m5C has been shown to participate in mRNA transport and maintain mRNA stability through its recognition by the reader proteins ALYREF and YBX1, respectively. We recently showed that m5C is required for Caenorhabditis elegans development and fertility under heat stress. To contribute to the understanding of how m5C and its oxidative derivatives mediate their functions, we developed RNA baits bearing modified cytosines in diverse structural contexts to pulldown potential readers in C. elegans. Our mass spectrometry analyses reveal unique binding proteins for each of the modifications. We validate our dataset by demonstrating that the nematode ALYREF homologues ALY-1 and ALY-2 preferentially bind m5C in vitro. The dataset presented here serves as an important scientific resource that will support the discovery of new functions of m5C and its derivatives. |
HostingRepository | PRIDE |
AnnounceDate | 2022-09-29 |
AnnouncementXML | Submission_2022-09-29_09:00:53.775.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Alejandro Brenes |
SpeciesList | scientific name: Caenorhabditis elegans; NCBI TaxID: 6239; |
ModificationList | acetylated residue; monohydroxylated residue; deamidated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-08-02 10:47:05 | ID requested | |
⏵ 1 | 2022-09-29 09:00:54 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Caenorhabditis elegans, c elegans, label free |
Contact List
Angus I. Lamond |
contact affiliation | Centre for Gene Regulation and Expression |
contact email | a.i.lamond@dundee.ac.uk |
lab head | |
Alejandro Brenes |
contact affiliation | University of Dundee |
contact email | ajbrenesmurillo@dundee.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD035761
- Label: PRIDE project
- Name: Identification of putative reader proteins of RNA 5-methylcytosine and its derivatives in Caenorhabditis elegans