PXD035665

PXD035665 is an original dataset announced via ProteomeXchange.

Title	Deep single-shot nanoLCMS proteome profiling with 1500 bar UHPLC system, long fully porous columns, and HRAM MS
Description	This study demonstrates how the latest UHPLC (ultra-high-performance liquid chromatography) technology can be combined with high-resolution accurate-mass (HRAM) mass spectrometry (MS) and long columns packed with fully porous particles to improve bottom-up proteomics analysis with nano-flow liquid chromatography mass-spectrometry (nanoLCMS) methods. The increased back pressures from the UHPLC system enabled the use of 75 µm I.D. x 75 cm columns packed with 2 µm particles at a typical 300 nL/min flow rate as well as elevated and reduced flow rates. The constant pressure pump operation at 1500 bar reduced sample loading and column washing/equilibration stages and overall overhead time that maximizes MS utilization time. The versatility of flow rate optimization to balance the sensitivity, throughput with sample loading amount, and capability of using longer gradients contribute to a greater number of peptide and protein identifications for single-shot bottom-up proteomics experiments. The routine proteome profiling and precise quantification of >7,000 proteins with single-shot nanoLC-MS analysis open possibilities for large-scale discovery studies with deep dive into the protein level alterations.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:57:47.213.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alexander Boychenko
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos; Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-01 02:37:43	ID requested
1	2022-09-12 12:59:53	announced
2	2023-05-14 15:06:12	announced	2023-05-14: Updated project metadata.
⏵ 3	2023-11-14 08:57:47	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

ProteomeXchange project tag: EPIC-XS

submitter keyword: Human, LCMS, cells, Orbitrap

Runsheng Zheng
contact affiliation	Thermo Fisher Scientific
contact email	runsheng.zheng@thermofisher.com
lab head
Alexander Boychenko
contact affiliation	Thermo Fisher Scientific
contact email	oleksandr.boychenko@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/09/PXD035665

PRIDE project URI

• PRIDE
  1. PXD035665
     1. Label: PRIDE project
     2. Name: Deep single-shot nanoLCMS proteome profiling with 1500 bar UHPLC system, long fully porous columns, and HRAM MS