PXD035478 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Immunization of mice with virus-like vesicles of Kaposi sarcoma-associated herpesvirus reveals a role of ORF4 in complement-mediated neutralization |
Description | The development of a prophylactic vaccine for Kaposi sarcoma-associated Herpesvirus (KSHV) would prevent consequences from infection including disorders such as Kaposi sarcoma and primary effusion lymphoma. Here, we study the immunogenicity of noninfectious virus-like vesicles (VLVs) of KSHV as a potential future vaccine platform. VLVs present a repertoire of viral structural proteins but are noninfectious due to a defect in capsid formation that prevents viral DNA packaging. Immunization of mice with adjuvanted VLVs results in virus-specific antibodies and T cells. These antibodies neutralize viral infection, and this neutralization is enhanced by the complement system. Complement-enhanced neutralization is dependent on antibodies targeting the SCR region of viral ORF4. However, this activity was not present in serum from KSHV-infected humans. Our study highlights an important role of antibody effector functions in the development of a future KSHV vaccine |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:41:54.386.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jeffrey Johnson |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Human gammaherpesvirus 8; NCBI TaxID: NCBITaxon:37296; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Eclipse |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-07-21 03:40:15 | ID requested | |
1 | 2023-02-01 10:37:19 | announced | |
⏵ 2 | 2024-10-22 05:41:54 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: KSHV herpesvirus |
Contact List
Jeffrey Johnson |
contact affiliation | microbiology department at the Icahn School of Medicine at Mount Sinai |
contact email | jeffrey.johnson@mssm.edu |
lab head | |
Jeffrey Johnson |
contact affiliation | Icahn School of Medicine at Mount Sinai |
contact email | jeffrey.johnson@mssm.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD035478
- Label: PRIDE project
- Name: Immunization of mice with virus-like vesicles of Kaposi sarcoma-associated herpesvirus reveals a role of ORF4 in complement-mediated neutralization