<<< Full experiment listing

PXD035478

PXD035478 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleImmunization of mice with virus-like vesicles of Kaposi sarcoma-associated herpesvirus reveals a role of ORF4 in complement-mediated neutralization
DescriptionThe development of a prophylactic vaccine for Kaposi sarcoma-associated Herpesvirus (KSHV) would prevent consequences from infection including disorders such as Kaposi sarcoma and primary effusion lymphoma. Here, we study the immunogenicity of noninfectious virus-like vesicles (VLVs) of KSHV as a potential future vaccine platform. VLVs present a repertoire of viral structural proteins but are noninfectious due to a defect in capsid formation that prevents viral DNA packaging. Immunization of mice with adjuvanted VLVs results in virus-specific antibodies and T cells. These antibodies neutralize viral infection, and this neutralization is enhanced by the complement system. Complement-enhanced neutralization is dependent on antibodies targeting the SCR region of viral ORF4. However, this activity was not present in serum from KSHV-infected humans. Our study highlights an important role of antibody effector functions in the development of a future KSHV vaccine
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:41:54.386.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJeffrey Johnson
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Human gammaherpesvirus 8; NCBI TaxID: NCBITaxon:37296;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Eclipse
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-07-21 03:40:15ID requested
12023-02-01 10:37:19announced
22024-10-22 05:41:54announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: KSHV herpesvirus
Contact List
Jeffrey Johnson
contact affiliationmicrobiology department at the Icahn School of Medicine at Mount Sinai
contact emailjeffrey.johnson@mssm.edu
lab head
Jeffrey Johnson
contact affiliationIcahn School of Medicine at Mount Sinai
contact emailjeffrey.johnson@mssm.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/02/PXD035478
PRIDE project URI
Repository Record List
[ + ]