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PXD035462

PXD035462 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentification of novel kinases of Tau using fluorescence complementation mass spectrometry (FCMS)
DescriptionHyperphosphorylation of the microtubule-associated protein Tau is a major hallmark of Alzheimer’s disease (AD) and other tauopathies. Understanding the protein kinases that phosphorylate Tau is critical for the development of new drugs that target Tau phosphorylation. At present, the repertoire of the Tau kinases remains incomplete, and methods to uncover novel upstream protein kinases are still limited. Here, we apply our newly developed proteomic strategy, fluorescence complementation mass spectrometry (FCMS), to identify novel kinase candidates of Tau. By constructing Tau- and kinase-fluorescent fragment library, we detected 59 Tau-associated kinases, including 23 known kinases of Tau and 36 novel candidate kinases. In the validation phase using in vitro phosphorylation, four kinase candidates, OXSR1, DAPK2, CSK, and ZAP70, displayed the ability to phosphorylate Tau. Furthermore, co-expression of these four kinases along with Tau increased the phosphorylation of Tau in human neuroglioma H4 cells. Taken together, we demonstrate the proteomic strategy to systematically identify potential kinases that can phosphorylate Tau in cells. Our discovery of new candidate kinases of Tau can present new opportunities for developing AD therapeutic strategies.
HostingRepositoryjPOST
AnnounceDate2023-07-21
AnnouncementXMLSubmission_2023-07-20_08:00:05.107.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterDer-Shyang Kao
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListS-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide; TMT6plex reporter+balance reagent N-acylated residue; TMT6plex reporter+balance reagent acylated N-terminal; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine
Instrumentinstrument; instrument; instrument
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-07-20 18:50:11ID requested
12023-07-20 08:00:05announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: kinases, Tau
Contact List
W. Andy Tao
lab head
Der-Shyang Kao
contact affiliationPurdue University
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
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