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PXD035451

PXD035451 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSARS-CoV-2 hijacks p38β/MAPK11 to promote virus replication
DescriptionSARS-CoV-2, the causative agent of the COVID-19 pandemic, drastically modifies infected cells in an effort to optimize virus replication. Included in this process is the activation of the host p38 mitogen-activated protein kinase (MAPK) pathway, which plays a major role in inflammation and is a central driver of COVID-19 clinical presentations. Inhibition of p38/MAPK activity in SARS-CoV-2-infected cells reduces both cytokine production and viral replication. Here, we combined genetic screening with quantitative phosphoproteomics to better understand interactions between the p38/MAPK pathway and SARS-CoV-2. We found several components of the p38/MAPK pathway impact SARS-CoV-2 replication and that p38β is a critical host factor that promotes viral replication and prevents activation of the interferon pathway. Quantitative phosphoproteomics uncovered several SARS-CoV-2 nucleocapsid (N) protein phosphorylation sites near the N-terminus that were sensitive to p38 inhibition. Similar to p38β depletion, mutation of these N residues was associated with reduced viral replication and increased activation of Type I interferon signaling. Taken together, this study reveals a unique proviral function for p38β that is not shared with p38α, and supports efforts to develop p38β inhibitors as a strategy towards developing a new class of COVID-19 therapies. methods
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:46:15.205.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJeffrey Johnson
SpeciesList scientific name: Severe acute respiratory syndrome coronavirus 2; NCBI TaxID: NCBITaxon:2697049; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListphosphorylated residue
InstrumentOrbitrap Eclipse
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-07-20 04:53:55ID requested
12023-06-01 08:43:11announced
22024-10-22 05:46:15announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
ProteomeXchange project tag: Sars-cov-2, Covid-19
submitter keyword: betacoronavirus, p38, MAPK11, MAPK, p38β, p38beta, phosphoproteomics, N, nucleocapsid,SARS-CoV-2, proteomics, coronavirus, COVID-19
Contact List
Jeffrey R. Johnson
contact affiliationDepartment of Microbiology Icahn School of Medicine Mount Sinai
contact emailjeffrey.johnson@mssm.edu
lab head
Jeffrey Johnson
contact affiliationIcahn School of Medicine at Mount Sinai
contact emailjeffrey.johnson@mssm.edu
dataset submitter
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Dataset FTP location
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