PXD035394

PXD035394 is an original dataset announced via ProteomeXchange.

Title	Nuclear myosin VI maintains replication fork stability
Description	The actin cytoskeleton is of fundamental importance for cellular structure and plasticity. However, abundance and function of filamentous (F-) actin in the nucleus are still controversial. Here we show that the actin-based molecular motor myosin VI contributes to the stabilization of stalled or reversed replication forks. In response to DNA replication stress, myosin VI associates with stalled replication intermediates and cooperates with the AAA ATPase WRNIP1 in protecting these structures from DNA2-mediated nucleolytic attack. Using functionalized DARPins to manipulate myosin VI levels in a compartment-specific manner, we provide evidence for the direct involvement of myosin VI in the nucleus and against a contribution of the abundant cytoplasmic pool during the replication stress response.
HostingRepository	PRIDE
AnnounceDate	2023-05-04
AnnouncementXML	Submission_2023-05-04_02:51:44.715.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	PetraBeli
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-20 00:09:50	ID requested
⏵ 1	2023-05-04 02:51:45	announced

Dataset with its publication pending

submitter keyword: Myosin VI, WRNIP1, DARPins, fork stability,DNA replication stress

PetraBeli
contact affiliation	Institute of Molecular Biology, Ackermannweg 4, 55128 Mainz, Germany
contact email	p.beli@imb-mainz.de
lab head
PetraBeli
contact affiliation	Institute of Molecular Biology (IMB), Mainz, Germany
contact email	p.beli@imb-mainz.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD035394
     1. Label: PRIDE project
     2. Name: Nuclear myosin VI maintains replication fork stability