PXD035078 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Evaluation of absorbent cotton for glycopeptide enrichment |
Description | This study introduced the use of cotton in 200µL tips, namely cotton HILIC as a convenient tool for enriching large-scale glycopeptides before MS analysis in this study. The optimal loading buffer for cotton HILIC was investigated by using mouse brain as a complex test sample. Subsequently, the performance of cotton HILIC was evaluated by comparing with other two commercial enrichment approaches, venusil HILIC and Oasis MAX in both mouse brain and seminal plasma. Moreover, capacity and recovery rate were evaluated using different milligram of cotton in 200µL tips. Thus, a simple, convenient, and cost-friendly cotton HILIC method was proposed to achieve highly selective intact glycopeptide profiling. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:56:07.826.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Miaomiao Xin |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | carbamoylated residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-07-04 14:58:55 | ID requested | |
1 | 2023-03-04 01:44:54 | announced | |
⏵ 2 | 2023-11-14 08:56:08 | announced | 2023-11-14: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: HILIC,Glycopeptide, Enrichment Absorbent cotton, Mass spectrometry, MAX |
Contact List
Shisheng Sun |
contact affiliation | Northwest University |
contact email | suns@nwu.edu.cn |
lab head | |
Miaomiao Xin |
contact affiliation | College of Life Science, Northwest University, Xi’an, Shaanxi Province 710069, P. R. China |
contact email | xinmiao1206@126.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD035078
- Label: PRIDE project
- Name: Evaluation of absorbent cotton for glycopeptide enrichment