PXD035001

PXD035001 is an original dataset announced via ProteomeXchange.

Title	Heat shock chaperone HSPB1 regulates cytoplasmic TDP-43 phase separation and liquid-to-gel transition
Description	While acetylated, RNA binding deficient TDP-43 reversibly phase separates within nuclei into complex droplets (anisosomes) comprised of TDP-43-containing liquid outer shells and liquid centers of HSP70 family chaperones, cytoplasmic aggregates of TDP-43 are hallmarks of multiple neurodegenerative diseases, including ALS. Here we show that transient oxidative stress, proteasome inhibition, or inhibition of HSP70’s ATP-dependent chaperone activity provokes reversible cytoplasmic TDP-43 de-mixing and transition from liquid to gel/solid, independent of RNA binding or stress granules. Proxmity labeling coupled with quantitative mass spectrometry is used to identify that phase separated cytoplasmic TDP-43 is bound by the small heat shock protein HSPB1.
HostingRepository	PRIDE
AnnounceDate	2022-08-09
AnnouncementXML	Submission_2022-08-09_07:36:59.677.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shan Lu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-30 03:15:52	ID requested
⏵ 1	2022-08-09 07:37:00	announced

Dataset with its publication pending

submitter keyword: Human, TDP-43, proximity labeling

John R. Yates III
contact affiliation	The Scripps Research Institute, La Jolla, CA 92037, USA
contact email	jyates@scripps.edu
lab head
Shan Lu
contact affiliation	Department of Cellular and Molecular Medicine
contact email	shl563@health.ucsd.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD035001
     1. Label: PRIDE project
     2. Name: Heat shock chaperone HSPB1 regulates cytoplasmic TDP-43 phase separation and liquid-to-gel transition