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PXD034805

PXD034805 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA mass spectrometry-based approach for the identification of Kpnβ1 binding partners in cancer cells
DescriptionKaryopherin beta 1 (Kpnβ1) is the principal nuclear importer of cargo proteins and plays a role in many cellular processes. Its expression is upregulated in cancer and essential for cancer cell viability, thus the identification of its binding partners might help in the discovery of anti-cancer therapeutic targets and cancer biomarkers. Herein, we applied immunoprecipitation coupled to mass spectrometry (IP- MS) to identify Kpnβ1 binding partners in normal and cancer cells. IP-MS identified 100 potential Kpnβ1 binding partners in non-cancer hTERT-RPE1, 179 in HeLa cervical cancer, 147 in WHCO5 oesophageal cancer and 176 in KYSE30 oesophageal cancer cells, including expected and novel interaction partners. 38 binding proteins were identified in all cell lines, with the majority involved in RNA metabolism. 18 binding proteins were unique to the cancer cells, with many involved in protein translation. Western blot analysis validated the interaction of known and novel binding partners with Kpnβ1 and revealed enriched interactions between Kpnβ1 and select proteins in cancer cells, including proteins involved in cancer development, such as Kpnα2, Ran, Crm1, CCAR1 and FUBP1. Together, this study shows that Kpnβ1 interacts with numerous proteins, and its enhanced interaction with certain proteins in cancer cells likely contributes to the cancer state.
HostingRepositoryPRIDE
AnnounceDate2022-11-03
AnnouncementXMLSubmission_2022-11-03_02:26:01.001.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterShaunGarnett
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListcarbamoylated residue; acetylated residue; monohydroxylated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-06-21 15:05:58ID requested
12022-11-03 02:26:01announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Cancer,KPNB1
Contact List
Virna DLeaner
contact affiliationSAMRC Gynaecology Cancer Research Centre, University of Cape Town, South Africa
contact emailvirna.leaner@uct.ac.za
lab head
ShaunGarnett
contact affiliationUniversity of Cape Town
contact emailshaungarnett@gmail.com
dataset submitter
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Dataset FTP location
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