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PXD034635

PXD034635 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProtein nonadditivity and stability contribute to heterosis in Arabidopsis hybrids and allotetraploids
DescriptionHybrid vigor or heterosis has been widely applied in agriculture and extensively studied at the genetic and gene expression levels. However, the biochemical mechanism underlying heterosis remains elusive. One theory suggests that a decrease in protein aggregation may occur in hybrids due to the presence of protein variants between parental alleles, but it has not been experimentally tested. Here, we report comparative analysis of soluble and insoluble proteomes in Arabidopsis intraspecific and interspecific hybrids or allotetraploids formed between A. thaliana and A. arenosa. Both intraspecific hybrids and interspecific allotetraploids displayed non-additivity of the expressed proteins, including many biotic and abiotic stress-responsive proteins. In the allotetraploids, homoeolog-expression bias was not observed among all proteins examined but could occur among 17-20% of the nonadditively expressed proteins with more A. thaliana-biased than A. arenosa-biased homoeologs, consistent with the transcriptome results. Analysis of the insoluble and soluble proteomes revealed more soluble proteins in the hybrids than their parents but not in allotetraploids. Most proteins in ribosomal biosynthesis and in the thylakoid lumen, membrane, and stroma, were in the soluble fractions, indicating a role of protein stability in photosynthetic activities for promoting growth. Together, these results support roles for nonadditive expression of stress-responsive proteins and reduction of protein biosynthesis in mediating heterosis in Arabidopsis hybrids and allotetraploids.
HostingRepositoryMassIVE
AnnounceDate2023-07-07
AnnouncementXMLSubmission_2023-07-07_19:21:27.565.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterViviana June
SpeciesList scientific name: Arabidopsis thaliana; common name: thale cress; NCBI TaxID: 3702; scientific name: Arabidopsis suecica; NCBI TaxID: 45249; scientific name: Arabidopsis arenosa; NCBI TaxID: 38785;
ModificationListOxidation; Carbamidomethyl
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-06-17 10:01:27ID requested
12023-07-07 19:21:27announced
Publication List
no publication
Keyword List
submitter keyword: Hybrid vigour, Heterosis, Arabidopsis, Allopolyploids, Protein solubility
Contact List
Z. Jeffrey Chen
contact affiliationUniversity of Texas at Austin
contact emailzjchen@austin.utexas.edu
lab head
Viviana June
contact affiliationUT Austin
contact emailvivianajune@utexas.edu
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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