PXD034355 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Phosphoproteomic analysis of neoadjuvant breast cancer reveals a subset of patients with increased sensitivity to paclitaxel driven by CDK4 and Filamin A. |
Description | Precision oncology research is challenging outside contexts of oncogenic addiction and/or targeted therapies. We previously showed that phosphoproteomics is a powerful approach to reveal patient subsets of interest characterized by the activity of a few kinases where the underlying genomics is complex. Here, we conducted a phosphoproteomic screening in a neoadjuvant trial of HER2-negative breast cancer patients (N=130) treated with paclitaxel or paclitaxel plus nintedanib, aiming to find candidate biomarkers of paclitaxel sensitivity. Filtering 15 candidate biomarkers through 2 independent patient sets (N=218) allowed finding a subgroup of patients characterized by high levels of CDK4 and Filamin-A, who had a 90% chance of achieving a pCR in response to paclitaxel. Mechanistically, CDK4 regulated Filamin-A transcription, which in turn formed a complex with Tubulin and CLIP-170, what elicited increased binding of paclitaxel to microtubules, microtubule acetylation and stabilization, and mitotic catastrophe. Thus, phosphoproteomics allowed finding explainable predictive factors for paclitaxel. |
HostingRepository | PRIDE |
AnnounceDate | 2022-11-08 |
AnnouncementXML | Submission_2022-11-08_04:10:00.898.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | MiguelQuintela |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-06-06 02:19:52 | ID requested | |
⏵ 1 | 2022-11-08 04:10:01 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: breast cancer, Filamin A,Phosphoproteomics, CDK4, paclitaxel |
Contact List
MiguelQuintela-Fandino |
contact affiliation | Breast Cancer Clinical Research Unit Centro Nacional de Investigaciones Oncológicas – CNIO Madrid, Spain. |
contact email | mquintela@cnio.es |
lab head | |
MiguelQuintela |
contact affiliation | Spanish National Cancer Research Centre, Spain |
contact email | mquintela@cnio.es |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/11/PXD034355 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD034355
- Label: PRIDE project
- Name: Phosphoproteomic analysis of neoadjuvant breast cancer reveals a subset of patients with increased sensitivity to paclitaxel driven by CDK4 and Filamin A.