PXD034273
PXD034273 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Isolation of intact and active FoF1 ATP synthase using a FLAG-tagged subunit from the cyanobacterium Synechocystis sp. PCC 6803 |
Description | The FoF1 ATP synthase (ATPase) is one of the most important protein complexes in the energy metabolism, with many open questions about its assembly, regulation, and functions. The isolation of functional ATPase complexes is fundamental for their subsequent biochemical and structural analysis. This protocol describes the acquisition of intact and active ATPase with good purity from Synechocystis sp. PCC 6803, based on a 3xFLAG tag fused to the beta subunit. The reproducibility of this protocol is high. |
HostingRepository | MassIVE |
AnnounceDate | 2022-07-19 |
AnnouncementXML | Submission_2022-07-19_02:18:20.087.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Stefan Tholen |
SpeciesList | scientific name: Synechocystis sp. PCC 6803; NCBI TaxID: 1148; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive Plus |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2022-06-03 03:14:27 | ID requested | |
⏵ 1 | 2022-07-19 02:18:20 | announced |
Publication List
no publication |
Keyword List
submitter keyword: ATPase, Synechocystis, cyanobacteria |
Contact List
Wolfgang R. Hess | |
---|---|
contact affiliation | Genetics and Experimental Bioinformatics, Faculty of Biology, University of Freiburg |
contact email | wolfgang.hess@biologie.uni-freiburg.de |
lab head | |
Stefan Tholen | |
contact affiliation | Uniklinik Freiburg |
contact email | stefan.tholen@uniklinik-freiburg.de |
dataset submitter |
Full Dataset Link List
MassIVE dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000089593/ |