PXD034242

PXD034242 is an original dataset announced via ProteomeXchange.

Title	The non-classical major-histocompatibility-complex II protein SLA-DM is crucial for African swine fever virus replication
Description	African swine fever virus (ASFV) is a lethal animal pathogen which enters its host cells through endocytosis. So far, host factors specifically required for ASFV replication have been barely identified. In this study a genome-wide CRISPR/Cas9 knockout screen in porcine cells indicated that the genes RFXANK, RFXAP, SLA-DMA, SLA-DMB, and CIITA are important for productive ASFV infection. The proteins encoded by these genes belong to the major-histocompatibility-complex II (MHC II), or swine-leucocyte-antigen-complex II (SLA II). RFXAP and CIITA are MHC II-specific transcription factors, whereas SLA-DMA/B are subunits of the non-classical MHC II molecule SLA-DM. Targeted knockout of either of these genes led to severe replication defects of different ASFV isolates, reflected by substantially reduced plating efficiency, cell-to-cell spread, and progeny virus titers. For the characterization of the knockouts on a proteome level the protein contents of the knockout cell lines were analyzed by mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:08:28.901.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Axel Karger
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	maXis

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-01 05:00:30	ID requested
1	2023-08-21 06:27:52	announced
⏵ 2	2023-11-14 07:08:29	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: SLA-DMB, African swine fever virus (ASFV), Major histocompatibility complex II (MHC II), SLA-DMA,Genome-wide CRISPR/Cas9 knockout screening (GeCKo), Swine leucocyte antigen complex II (SLA II)

Axel Karger
contact affiliation	Institute of molecular virology and cell biology
contact email	axel.karger@fli.de
lab head
Axel Karger
contact affiliation	Friedrich-Loeffler-Institut
contact email	axel.karger@fli.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/08/PXD034242

PRIDE project URI

• PRIDE
  1. PXD034242
     1. Label: PRIDE project
     2. Name: The non-classical major-histocompatibility-complex II protein SLA-DM is crucial for African swine fever virus replication