PXD034170
PXD034170 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Bioprospecting the American Alligator Peptidome for Antiviral Peptides Against Venezuelan Equine Encephalitis Virus |
| Description | The innate immune protection provided by cationic antimicrobial peptides (CAMPs) has been shown to extend to antiviral activity, with putative mechanisms of action including direct interaction with host cells or pathogen membranes. The lack of therapeutics available for the treatment of viruses such as Venezuelan equine encephalitis virus (VEEV) underscores the urgency of novel strategies for antiviral discovery. American alligator plasma has been shown to exhibit strong in vitro antibacterial activity, and functionalized hydrogel particles have been successfully employed for the identification of specific CAMPs from alligator plasma. Here, a novel bait strategy in which particles were encapsulated in membranes from either healthy or VEEV-infected cells was implemented to identify peptides preferentially targeting infected cells for subsequent evaluation of antiviral activity. Statistical analysis of peptide identification results was used to select five candidate peptides for testing, of which three exhibited a dose-dependent inhibition of VEEV, with one peptide also significantly inhibiting infectious titers. Results suggest our bioprospecting strategy provides a versatile platform that may be adapted for antiviral peptide identification from complex biological samples. |
| HostingRepository | MassIVE |
| AnnounceDate | 2022-12-14 |
| AnnouncementXML | Submission_2022-12-14_10:37:37.806.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Amy Carfagno |
| SpeciesList | scientific name: Alligator mississippiensis; common name: American alligator; NCBI TaxID: 8496; |
| ModificationList | Oxidation; Acetyl; Amidated |
| Instrument | Orbitrap Fusion ETD |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2022-05-27 12:29:56 | ID requested | |
| ⏵ 1 | 2022-12-14 10:37:38 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: antiviral peptides, membrane-encapsulated particles, non-model organism, Venezuelan equine encephalitis virus |
Contact List
| Barney Bishop | |
|---|---|
| contact affiliation | George Mason University |
| contact email | bbishop1@gmu.edu |
| lab head | |
| Amy Carfagno | |
| contact affiliation | George Mason University |
| contact email | acarfagn@gmu.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000089560/ |
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