PXD034041

PXD034041 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Apically-located P4-ATPase1-Lem1 complex flips phosphatidylserine and regulates motility-dependent invasion and egress in Toxoplasma gondii
Description	The membrane asymmetry regulated by P4-ATPases in crucial for optimal functioning of eukaryotic cells. The underlying spatial translocation or flipping of specific lipids is usually assured by respective P4-ATPases coupled to conforming non-catalytic subunits. Our previous work has identified five P4-ATPases (TgP4-ATPase1-5) and three partner proteins (TgLem1-3) in the intracellular protozoan pathogen, Toxoplasma gondii. However, their flipping activity, physiological relevance and functional coupling remain unknown. Herein, we demonstrate that TgP4-ATPase1 and TgLem1 work together to translocate phosphatidylserine (PtdSer) during the lytic cycle of T. gondii. The two proteins localize in the plasma membrane at the invasive (apical) end of its acutely-infectious tachyzoites stage. The knockout of P4-ATPase1 as well as depletion of Lem1 severely disrupt the asexual reproduction of tachyzoites along with their ability of translocate PtdSer. Moreover, phenotypic analysis of individual mutants revealed their requirement for the motility, egress and invasion of tachyzoites. Our findings disclose the significance and mechanism of PtdSer flipping during the lytic cycle and emphasize P4-ATPase1/Lem1 heterocomplex as a potential drug target in T. gondii.
HostingRepository	jPOST
AnnounceDate	2023-05-25
AnnouncementXML	Submission_2023-05-24_08:00:08.897.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ute Distler
SpeciesList	scientific name: cellular organisms; NCBI TaxID: 131567;
ModificationList	S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide
Instrument	instrument

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-24 10:28:15	ID requested
⏵ 1	2023-05-24 08:00:09	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: Toxoplasma gondii, Exploris480, proximity labeling, P4-ATPase1, Lem1, protein-protein intercation

submitter keyword: Toxoplasma gondii, Exploris480, proximity labeling, P4-ATPase1, Lem1, protein-protein intercation

Contact List

Nishith Gupta
lab head
Ute Distler
contact affiliation	University Medical Center Mainz
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001600/