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PXD034026

PXD034026 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA cell type-specific proteomics uncovers a guard cell-specific RAF15-SnRK2.6/OST1 kinase cascade
DescriptionMulticellular organisms such as plants contain different cell types with specialized functions. Analyzing the characteristics of each cell type reveals specific cell functions and enhances understanding of organization and function at the organismal level. Here we used enzymatic isolation and flow cytometry-activated cell sorting (FACS) to enrich the guard cell and mesophyll cell protoplasts and acquire in-depth proteomics in these two major cell types in Arabidopsis leaves. We uncovered a guard cell-specific kinase cascade whereby RAF15 and OST1 mediate ABA-induced stomatal closure. This pipeline can be applied to various cell types in plant or non-plant systems to learn how cells function in highly organized multicellular organisms.
HostingRepositoryjPOST
AnnounceDate2023-05-23
AnnouncementXMLSubmission_2023-05-22_08:00:04.436.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterHongliang Wang
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationListS-carboxymethyl-L-cysteine; alpha-amino acetylated residue
InstrumentOrbitrap Fusion; instrument
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-05-23 03:33:38ID requested
12023-05-22 08:00:04announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: proteomics, single-cell, guard cell, abscisic acid, phosphorylation
Contact List
Pengcheng Wang
lab head
Hongliang Wang
contact affiliationShanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 200032, China
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
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