PXD033940 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | An optimized proteomics approach reveals novel alternative proteins in mouse liver development |
Description | Alternative open reading frames (AltORFs) are unannotated sequences in genome that encode novel peptides or proteins named alternative proteins (AltProts). Although ribosome profiling and bioinformatics predict a large number of AltProts, mass spectrometry (MS) as the only direct way of identification, is hampered by the short lengths and relative low abundance of AltProts. Here we report an approach based on size-exclusion chromatography (SEC) for simultaneous enrichment and fractionation of AltProts from mouse livers. With this strategy we compared and discovered 89 novel AltProts between embryonic and adult mouse livers and will extend our optimized workflow in various disease models in future study. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:56:08.101.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Ying YANG |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetic acid derivatized residue |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-05-17 08:28:28 | ID requested | |
1 | 2022-12-23 04:37:47 | announced | |
⏵ 2 | 2023-11-14 08:56:08 | announced | 2023-11-14: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: liver development., mass spectrometry, fractionation,Alternative open reading frames (AltORFs), alternative proteins (AltProts), enrichment |
Contact List
Qian Zhao |
contact affiliation | State Key Laboratory of Chemical Biology and Drug Discovery, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hong Kong, SAR, China |
contact email | q.zhao@polyu.edu.hk |
lab head | |
Ying YANG |
contact affiliation | the Hong Kong Polytechnic University |
contact email | 20033267r@connect.polyu.hk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/12/PXD033940 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD033940
- Label: PRIDE project
- Name: An optimized proteomics approach reveals novel alternative proteins in mouse liver development