PXD033843 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Duplicated ribosomal protein paralogs promote alternative translation and drug resistance |
Description | Ribosomes are often seen as monolithic machines produced from uniformly regulated genes. However, in yeast most ribosomal proteins come from duplicated genes. Here, we demonstrate that gene duplication may serve as a stress-adaptation mechanism modulating the global proteome through the differential expression of ribosomal protein paralogs. Our data indicate that the yeast paralog pair of the ribosomal protein L7/uL30 produces two differentially acetylated proteins. Under normal conditions most ribosomes incorporate the hypo-acetylated major form favoring the translation of genes with short open reading frames. Exposure to drugs, on the other hand, increases the production of ribosomes carrying the hyper-acetylated minor paralog that increases translation of long open reading frames. Many of these paralogs dependent genes encode cell wall proteins that could promote tolerance to drugs as their translation increases after exposure to drugs. Together the data reveal a mechanism of translation control through the differential use of near-identical ribosomal protein isoforms. |
HostingRepository | PRIDE |
AnnounceDate | 2022-08-09 |
AnnouncementXML | Submission_2022-08-09_11:50:42.301.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Mathieu Catala |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | carbamoylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-05-12 04:29:44 | ID requested | |
⏵ 1 | 2022-08-09 11:50:42 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Saccharomyces cerevisiae, dRPG, paralogs |
Contact List
Sherif Abou Elela |
contact affiliation | Universite de Sherbrooke |
contact email | sherif.abou.elela@usherbrooke.ca |
lab head | |
Mathieu Catala |
contact affiliation | Universite de Sherbrooke |
contact email | mathieu.catala@usherbrooke.ca |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD033843
- Label: PRIDE project
- Name: Duplicated ribosomal protein paralogs promote alternative translation and drug resistance