PXD033752

PXD033752 is an original dataset announced via ProteomeXchange.

Title	Salivary orosomucoid 1 as a biomarker of hepatitis B associated hepatocellular carcinoma
Description	Saliva is rich in proteins, DNA, RNA and microorganisms, and can be regarded as a biomarker library. In order to explore a noninvasive and simple means of early screening for liver cancer, proteomics was used to screen salivary markers of hepatitis B associated liver cancer. We used mass spectrometry coupled isobaric tags for relative and absolute quantitation (iTRAQ)-technology to identify differentially expressed proteins (DEPs). Western blot, immunohistochemistry and enzyme linked immunosorbent assay were used to detect marker expression of in tissues and saliva. Statistical analysis was used to analyze the diagnostic efficacy of the markers was analyzed through statistical analyses. By comparing the hepatocellular carcinoma (HCC) group with non-HCC groups, we screened out 152 salivary DEPs. We found orosomucoid 1(ORM1) had significantly higher expression in saliva of HCC patients compared with non-HCC groups (p<0.001) and the expression of ORM1 in liver cancer tissues was significantly higher than that in adjacent normal tissues(p<0.001). The combination of salivary ORM1 and alpha-fetoprotein (AFP) showed reasonable specificities and sensitivities for detecting HCC. In a word, salivary ORM1 as a new biomarker of hepatitis B associated hepatocellular carcinoma, combination of salivary ORM1 and AFP as an improved diagnostic tool for hepatocellular carcinoma.
HostingRepository	PRIDE
AnnounceDate	2022-07-13
AnnouncementXML	Submission_2022-07-13_04:50:22.261.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jiaoxia He
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-06 10:46:26	ID requested
⏵ 1	2022-07-13 04:50:22	announced

Dataset with its publication pending

submitter keyword: Salivary, Orosomucoid 1, α-fetoprotein, Hepatocellular carcinoma, Biomarkers

Huaidong Hu
contact affiliation	Department of Infectious Diseases, Key Laboratory of Molecular Biology for Infectious Diseases, Institute for Viral Hepatitis, the Second Affiliated Hospital of Chongqing Medical University
contact email	huhuaidong@cqmu.edu.cn
lab head
Jiaoxia He
contact affiliation	the Second Affiliated Hospital of Chongqing Medical University
contact email	1325381737@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD033752
     1. Label: PRIDE project
     2. Name: Salivary orosomucoid 1 as a biomarker of hepatitis B associated hepatocellular carcinoma