PXD033640 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Plasmid manipulation of bacterial behaviour through translational regulatory crosstalk |
Description | pQBR103 is a naturally occurring plasmid known to alter the behaviour of its host, Pseudomonas fluorescens. This plasmid encodes a predicted translational regulator, rsmQ which is a homologue of known global regulators within Pseudomonas. Within this study we compared Pseudomonas fluorescens SBW25 plasmid free cells with cells carrying either WT pQBR103 or pQBR103 lacking rsmQ. Using comparative quantitative proteomics and RNAseq we showed that the loss of rsmQ leads to widespread proteomic changes in the absence of changes in mRNA abundance, suggesting RsmQ is a translational regulator of its host chromosome. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:56:25.063.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Gerhard Saalbach |
SpeciesList | scientific name: Pseudomonas fluorescens (strain SBW25); NCBI TaxID: 216595; scientific name: Bacteria; NCBI TaxID: NCBITaxon:2; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Eclipse |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-05-04 14:39:56 | ID requested | |
1 | 2023-01-13 03:39:14 | announced | |
⏵ 2 | 2023-11-14 08:56:25 | announced | 2023-11-14: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: TMT, RNAseq, translational regulator,Pseudomonas fluorescens SBW25, quantitative proteomics, plasmid |
Contact List
Jacob G Malone |
contact affiliation | Dept. Molecular Microbiology John Innes Centre Norwich Research Park Norwich, UK |
contact email | jacob.malone@jic.ac.uk |
lab head | |
Gerhard Saalbach |
contact affiliation | Biological Chemistry |
contact email | gerhard.saalbach@jic.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD033640
- Label: PRIDE project
- Name: Plasmid manipulation of bacterial behaviour through translational regulatory crosstalk