PXD033583 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | RcgA and RcgR, two novel proteins involved in conjugative transfer of rhizobial plasmids |
Description | Rhizobia are gram-negative bacteria able to establish a symbiotic interaction with leguminous plants. Due to their nitrogen fixing capacity, the study of these microorganisms has acquired great relevance for the agriculture. Rhizobia usually harbor many plasmids in their genome which can be transferred to other organisms by conjugation. Two main mechanisms of regulation of rhizobial plasmid transfer have been described: Quorum sensing (QS) and rctA/rctB system. Nevertheless, new genes and molecules that modulate conjugative transfer have been recently described, demonstrating that new actors can tightly regulate the process. In this work, by means of bioinformatics tools and molecular biology approaches, two hypothetical genes are identified as playing key roles in conjugative transfer. These genes are located between conjugative genes of plasmid pLPU83a from Rhizobium favelukesii LPU83, a plasmid that showed a conjugative transfer behavior depending on the genomic background. One of the two mentioned genes, rcgA, is essential for conjugation, while the other, rcgR, acts as an inhibitor of the process. In addition to introducing this new regulatory mechanism, we show evidence of the functions of these genes in different genomic backgrounds, and confirmed that homologous proteins from non-closely related organisms play the same function. These findings set up a cornerstone for a new molecular circuit of conjugative transfer of plasmids. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:56:23.412.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Gonzalo Torres Tejerizo |
SpeciesList | scientific name: Rhizobium favelukesii; NCBI TaxID: 348824; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive Plus |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-05-04 09:10:32 | ID requested | |
1 | 2022-08-29 05:53:09 | announced | |
⏵ 2 | 2023-11-14 08:56:23 | announced | 2023-11-14: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: conjugation,Rhizobia, plasmid |
Contact List
Gonzalo Torres Tejerizo |
contact affiliation | Instituto de Biotecnología y Biología Molecular Facultad de Ciencias Exactas. Universidad Nacional de La Plata. CCT La Plata CONICET Calle 49 y 115 1900. La Plata Argentina |
contact email | gatt@biol.unlp.edu.ar |
lab head | |
Gonzalo Torres Tejerizo |
contact affiliation | Instituto de Biotecnología y Biología Molecular Facultad de Ciencias Exactas. Universidad Nacional de La Plata. CCT La Plata CONICET Calle 49 y 115 1900. La Plata |
contact email | gatt@biol.unlp.edu.ar |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD033583
- Label: PRIDE project
- Name: RcgA and RcgR, two novel proteins involved in conjugative transfer of rhizobial plasmids