PXD033553

PXD033553 is an original dataset announced via ProteomeXchange.

Title	Role of the two-component system CiaRH in the regulation of efflux pump SatAB and its correlation with fluoroquinolones susceptibility
Description	Streptococcus suis is a zoonotic pathogen with a high incidence and mortality rate in both swine and humans. Following antibiotic treatment, the organism has evolved many resistance mechanisms, among of which efflux pump overexpression can promote drug extrusion out of the cell. This study clarified the role of CiaRH in fluoroquinolone resistance. A deletion of the ciaRH genes showed decreased susceptibility to tested antibiotics, an invariant growth rate, and reduced intracellular substrates. This research also demonstrated that the overexpression of efflux pump, SatAB, was the main cause of ∆ciaRH resistance. In addition, CiaR could combine with the promoter region of satAB, to further directly suppress target gene transcription. Simultaneously, satAB was also directly regulated by SatR. Our findings may provide novel insight into the development of drug targets, and help exploit corresponding inhibitors to combat bacterial multidrug resistance.
HostingRepository	PRIDE
AnnounceDate	2022-05-24
AnnouncementXML	Submission_2022-05-24_05:53:40.871.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wei Peng
SpeciesList	scientific name: Streptococcus suis SC84; NCBI TaxID: 568813;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-28 02:39:05	ID requested
⏵ 1	2022-05-24 05:53:41	announced

Dataset with its publication pending

submitter keyword: Streptococcus suis, two-component systems, fluoroquinolones, efflux pump, repressor

Weicheng Bei
contact affiliation	Huazhong Agricultural University
contact email	beiweic@163.com
lab head
Wei Peng
contact affiliation	Huazhong Agricultural University
contact email	pengweivet@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD033553
     1. Label: PRIDE project
     2. Name: Role of the two-component system CiaRH in the regulation of efflux pump SatAB and its correlation with fluoroquinolones susceptibility