PXD033394 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Angel2 phosphatase activity is required for non-canonical mitochondrial RNA processing |
Description | Canonical RNA processing in mammalian mitochondria is defined by tRNAs acting as recognition sites for nucleases to release flanking transcripts. The relevant factors, their structures, and mechanism are well described, but not all mitochondrial transcripts are punctuated by tRNAs, and their mode of processing has remained unsolved. Using Drosophila and mouse models, we demonstrate that non-canonical processing results in the formation of 3′ phosphates, and that phosphatase activity by the carbon catabolite repressor 4 domain-containing family member ANGEL2 is required for their hydrolysis. Furthermore, our data suggest that members of the FAST kinase domain-containing protein family are responsible for these 3′ phosphates. Our results therefore resolve the mechanism of non-canonical RNA processing in metazoan mitochondria, by defining the role of ANGEL2. |
HostingRepository | PRIDE |
AnnounceDate | 2022-09-01 |
AnnouncementXML | Submission_2022-09-01_07:46:37.596.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Florian Schober |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-04-23 15:33:22 | ID requested | |
⏵ 1 | 2022-09-01 07:46:37 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Mitochondria, RNA, processing, Angel2, Drosophila, Mouse |
Contact List
Anna Wredenberg |
contact affiliation | Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Solna/Sweden |
contact email | anna.wredenberg@ki.se |
lab head | |
Florian Schober |
contact affiliation | MMK, Karolinska Institute |
contact email | florian.schober@ki.se |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD033394
- Label: PRIDE project
- Name: Angel2 phosphatase activity is required for non-canonical mitochondrial RNA processing