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PXD033394

PXD033394 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAngel2 phosphatase activity is required for non-canonical mitochondrial RNA processing
DescriptionCanonical RNA processing in mammalian mitochondria is defined by tRNAs acting as recognition sites for nucleases to release flanking transcripts. The relevant factors, their structures, and mechanism are well described, but not all mitochondrial transcripts are punctuated by tRNAs, and their mode of processing has remained unsolved. Using Drosophila and mouse models, we demonstrate that non-canonical processing results in the formation of 3′ phosphates, and that phosphatase activity by the carbon catabolite repressor 4 domain-containing family member ANGEL2 is required for their hydrolysis. Furthermore, our data suggest that members of the FAST kinase domain-containing protein family are responsible for these 3′ phosphates. Our results therefore resolve the mechanism of non-canonical RNA processing in metazoan mitochondria, by defining the role of ANGEL2.
HostingRepositoryPRIDE
AnnounceDate2022-09-01
AnnouncementXMLSubmission_2022-09-01_07:46:37.596.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterFlorian Schober
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-04-23 15:33:22ID requested
12022-09-01 07:46:37announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Mitochondria, RNA, processing, Angel2, Drosophila, Mouse
Contact List
Anna Wredenberg
contact affiliationDepartment of Medical Biochemistry and Biophysics, Karolinska Institutet, Solna/Sweden
contact emailanna.wredenberg@ki.se
lab head
Florian Schober
contact affiliationMMK, Karolinska Institute
contact emailflorian.schober@ki.se
dataset submitter
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Dataset FTP location
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