PXD033234 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | CryoEM structure of mitochondrial complex I from the thermophilic eukaryote Chaetomium thermophilum |
Description | Mitochondrial complex I is a redox-driven proton pump that generates most of the proton-motive force powering oxidative phosphorylation and ATP synthesis in eukaryotes. We report the structure of complex I from the thermophilic eukaryote Chaetomium thermophilum, determined by electron cryo-microscopy to 2.4 A resolution in the open and closed conformation. Complex I has two arms, the peripheral and membrane arm, forming an L-shape. The two conformations differ in the relative position of the two arms. The open-to-closed transition is accompanied by substantial conformational changes in the Q-binding cavity and the E-channel, and by the formation of an aqueous connection between the E-channel and an extensive aqueous passage inside the membrane arm. The observed similarities provide strong support for a conserved, common mechanism that applies across all species from fungi to mammals. Furthermore, the complex is inhibited by the detergent DDM, which binds reversibly to two sites in the Q-binding cavity. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:54:12.796.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Julian Langer |
SpeciesList | scientific name: Chaetomium thermophilum; NCBI TaxID: 209285; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-04-14 05:07:32 | ID requested | |
1 | 2022-11-29 01:34:30 | announced | |
⏵ 2 | 2023-11-14 08:54:13 | announced | 2023-11-14: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: mitochondrial complex I, Chaetomium thermophilum |
Contact List
Julian Langer |
contact affiliation | Max Planck Institute of Biophysics, Max Planck Institute for Brain Research |
contact email | julian.langer@biophys.mpg.de |
lab head | |
Julian Langer |
contact affiliation | MPIs for Biophysics and Brain Research |
contact email | julian.langer@biophys.mpg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD033234
- Label: PRIDE project
- Name: CryoEM structure of mitochondrial complex I from the thermophilic eukaryote Chaetomium thermophilum