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PXD033155

PXD033155 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic insight into the interaction of Paenibacillus larvae with honey bee larvae before capping collected from an American foulbrood outbreak
DescriptionThe samples used in this study originated from colonies of managed European honey bees (A. mellifera) that were at the epicenter of an AFB outbreak in 2020. The colonies were officially examined by the State Veterinary Administration of the Czech Republic. For our analyses, we targeted brood samples recognized as larvae before capping. Samples from two sites, namely, the villages Nevsova (denoted by N) and Tetcice (denoted by T), are included in this study. We aimed to analyze samples of honey bee broods infected and affected by the pathogenic bacterium P. larvae. The colonies from which we collected samples had typical clinical signs of AFB. For our analysis, we selected larvae just before capping. We decided to analyze the samples using high-throughput proteomics, and to select an array of samples, it was necessary to confirm whether the samples were infected by P. larvae. Importantly, the presence/absence of P. larvae was confirmed using PCR before the proteomic analysis in the same individual sample. The study was supported by project no. QK1710228 (NAZV) and RO0418 of the Ministry of Agriculture of the Czech Republic.
HostingRepositoryMassIVE
AnnounceDate2022-08-18
AnnouncementXMLSubmission_2022-08-18_07:46:21.568.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterTomas Erban
SpeciesList scientific name: Apis mellifera; scientific name: Paenibacillus larvae;
ModificationListunknown modification; unknown modification; Carbamidomethyl
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-04-11 04:38:09ID requested
12022-08-18 07:46:21announced
Publication List
no publication
Keyword List
submitter keyword: Bacillus larvae, neutral metalloproteinase, label-free proteomics, host-pathogen interaction
Contact List
Tomas Erban
contact affiliationCrop Research Institute
contact emailarachnid@centrum.cz
lab head
Tomas Erban
contact affiliationCrop Research Institute
contact emailarachnid@centrum.cz
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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