PXD033045 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Nucleoporins facilitate ORC loading onto chromatin |
Description | The origin recognition complex (ORC) binds throughout the genome to initiate DNA replication. In metazoans, it is still unclear how ORC is targeted to specific loci to facilitate helicase loading and replication initiation. Here, we performed immunoprecipitations coupled with mass spectrometry for ORC2 in Drosophila embryos. Surprisingly, we found that ORC2 associates with multiple subunits of the Nup107-160 subcomplex of the nuclear pore. Bioinformatic analysis revealed that, relative to all modENCODE factors, nucleoporins are among the most enriched factors at ORC2 binding sites. Critically, depletion of the nucleoporin Elys, a member of the Nup107-160 complex, results in decreased ORC2 loading onto chromatin. Depleting Elys also sensitized cells to replication fork stalling, which could reflect a defect in establishing dormant replication origins. Our work reveals a new connection between ORC, replication initiation and nucleoporins, highlighting a previously unrecognized function of nucleoporins in metazoan replication initiation. |
HostingRepository | PRIDE |
AnnounceDate | 2022-10-19 |
AnnouncementXML | Submission_2022-10-19_09:51:46.941.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | LoganRichards |
SpeciesList | scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-04-07 02:55:06 | ID requested | |
⏵ 1 | 2022-10-19 09:51:47 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: DNA replication, Drosophila, genome stability, Nuclear Pore |
Contact List
JaredNordman |
contact affiliation | Department of Biological Sciences, Vanderbilt University |
contact email | jared.nordman@vanderbilt.edu |
lab head | |
LoganRichards |
contact affiliation | Vanderbilt University |
contact email | logan.r.richards@vanderbilt.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
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[ - ]
- PRIDE
- PXD033045
- Label: PRIDE project
- Name: Nucleoporins facilitate ORC loading onto chromatin