PXD033010 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Lung macrophages utilize unique cathepsin K-dependent phagosomal machinery to degrade intracellular collagen |
Description | Resident tissue macrophages (RTMs) are organ-specialized phagocytes responsible for the maintenance and protection of residing tissue. It is well established that tissue diversity is reflected by the heterogeneity of RTMs origin and phenotype. However, much less is known about tissue-specific phagocytic macrophage functions. Here, using quantitative proteomics approach, we identify cathepsins as key determinants of phagosome maturation in primary peritoneal, lung and brain resident macrophages. The data further uncover cathepsin K (CtsK) as a molecular marker for lung phagosomes required for intracellular protein and collagen degradation. Pharmacological blockade of CtsK activity diminished phagosomal proteolysis and collagenolysis in lung resident macrophages. Furthermore, pro-fibrotic TGF-β negatively regulated CtsK-mediated phagosomal collagen degradation independently from classical endocytic proteolytic pathways. In humans, phagosomal CtsK activity was reduced in lung COPD macrophages and lung macrophages exposed to cigarette smoke extract. Taken together, this study provides a comprehensive map of how peritoneal, lung and brain tissue environment shapes phagosomal composition of resident macrophages, revealing CtsK as a key molecular determinant of lung phagosomes contributing to phagocytic collagen clearance in lungs. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:55:20.215.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Ivo Fabrik |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-04-06 08:11:06 | ID requested | |
1 | 2023-01-17 23:47:38 | announced | |
⏵ 2 | 2023-11-14 08:55:20 | announced | 2023-11-14: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: phagocytosis, proteomics,Cathepsins, resident tissue macrophages, phagosome maturation |
Contact List
Anetta Härtlova |
contact affiliation | Institute of Biomedicine, Department of Microbiology, the Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden |
contact email | anetta.hartlova@gu.se |
lab head | |
Ivo Fabrik |
contact affiliation | Biomedical Research Center, University Hospital Hradec Kralove |
contact email | ivo.fabrik@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD033010
- Label: PRIDE project
- Name: Lung macrophages utilize unique cathepsin K-dependent phagosomal machinery to degrade intracellular collagen