PXD032954

PXD032954 is an original dataset announced via ProteomeXchange.

Title	mCherry contains a fluorescent protein isoform that interferes with its reporter function
Description	Fluorescent proteins are essential reporters in cell biology and molecular biology. Here, we reveal that red-fluorescent proteins possess an alternative translation initiation site that produces a short functional protein isoform. The short isoform creates significant background fluorescence that biases the outcome of expression studies. Our investigation identifies the short protein isoform, traces its origin, and determines the extent of the issue within the family of red fluorescent protein. Our analysis shows that the short isoform defect of the red fluorescent protein family may affect the interpretation of many published studies. Finally, we provide a re-engineered mCherry variant that lacks background expression as an improved tool for imaging and protein expression studies.
HostingRepository	PRIDE
AnnounceDate	2022-04-19
AnnouncementXML	Submission_2022-04-19_06:55:42.730.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Davi Fonseca
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-02 14:31:43	ID requested
⏵ 1	2022-04-19 06:55:43	announced

Dataset with its publication pending

submitter keyword: mCherry, LC-MSMS

Martin Hohmann-Marriott
contact affiliation	Department of Biotechnology, Norwegian University of Science and Technology, Norway. United Scientists CORE (Limited), Aotearoa - New Zealand.
contact email	martin.hohmann-marriott@ntnu.no
lab head
Davi Fonseca
contact affiliation	Norwegian University of Science and Technology
contact email	davi.fonseca@ntnu.no
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD032954

PRIDE project URI

• PRIDE
  1. PXD032954
     1. Label: PRIDE project
     2. Name: mCherry contains a fluorescent protein isoform that interferes with its reporter function