PXD032913 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Phosphoproteomics reveals rewiring of the insulin signaling network and multi-nodal defects in insulin resistance |
Description | Here we employed phosphoproteomics to delineate insulin signal transduction in adipocyte cells and adipose tissue. Across a range of insults triggering insulin resistance, we observed marked rewiring of the insulin signaling network. This included both attenuated insulin-responsive phosphorylation, and the emergence of phosphorylation uniquely insulin-regulated in insulin resistance. Identifying dysregulated phosphosites common to multiple insults revealed subnetworks containing novel regulators of insulin action, such as MARK2/3, and causal drivers of insulin resistance. The presence of several GSK3 substrates among these phosphosites led us to establish a pipeline for identifying context-specific kinase substrates. This revealed widespread dysregulation of GSK3 signaling. Pharmacological inhibition of GSK3 partially reversed insulin resistance in cells and tissue explants. These data highlight that insulin resistance is multi-nodal signaling defect that includes dysregulated GSK3 activity. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:55:35.473.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Sean Humphrey |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | phosphorylated residue |
Instrument | Q Exactive HF; Orbitrap Exploris 480; Q Exactive HF-X |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-04-02 11:42:32 | ID requested | |
1 | 2023-02-17 09:45:38 | announced | |
⏵ 2 | 2023-11-14 08:55:36 | announced | 2023-11-14: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: post-translational modification, adipocyte, mass spectrometry, fat, phosphoproteomics, GSK3, tissue, proteomics, adipose, signal transduction, phosphorylation, insulin signalling,insulin resistance |
Contact List
Sean Humphrey |
contact affiliation | The University of Sydney, Australia |
contact email | sean.j.humphrey@gmail.com |
lab head | |
Sean Humphrey |
contact affiliation | Murdoch Children's Research Institute |
contact email | sean.j.humphrey@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/02/PXD032913 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD032913
- Label: PRIDE project
- Name: Phosphoproteomics reveals rewiring of the insulin signaling network and multi-nodal defects in insulin resistance