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PXD032794

PXD032794 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAntimicrobial potential of a ponericin-like peptide isolated from Bombyx mori L. hemolymph in response to Pseudomonas aeruginosa infection
DescriptionThe main effectors in innate immune system of B. mori L. are antimicrobial peptides (AMPs). We imunizied B. mori with varied inoculum size of Pseudomonas aeruginosa ATCC 25668 cells to relate the importance of inoculum size towards AMP production. There is a sharp change in λmax from 278 nm to 285 nm (bathochromic shift) in the hemolymph of immunized silkworm incubated for 24 h. FTIR study of the hemolymph extracted from the immunized B. mori showed peak at 1550 cm-1 indicates the presence of α-helical peptides in the hemolymph.The peptide fraction was obtained through methanol, acetic acid and water mixture (90:1:9) extraction, followed by peptide purification using RP-HPLC. The fraction exhibiting antibacterial property was collected and characterized further using MALDI-TOF mass spectrometry. A linear -helical peptide with flexible termini (LLKELWTKMKGAGKAVLGKIKGLL -Bm-Frac6), corresponding to a previously described peptide from ant venom and denominated ponericin-L1. This peptide showed antibacterial activity against P. aeruginosa. Moreover, MTT assay and hemolytic study revealed that the Bm-Frac6 (B. mori ponericin-L1) is non-toxic toward mammalian healthy cells. Finally, our study shows that silkworm larvae challenged with P. aeruginosa also induced modification in physiological processes, leading to significant morpho-anatomical changes.
HostingRepositoryPRIDE
AnnounceDate2022-06-23
AnnouncementXMLSubmission_2022-06-22_23:04:35.140.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAHMET KATI
SpeciesList scientific name: Bombyx mori (Silk moth); NCBI TaxID: 7091;
ModificationListNo PTMs are included in the dataset
Instrumentultraflex
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-03-24 13:42:41ID requested
12022-06-22 23:04:35announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: ponericin, antimicrobial peptide, Bombyx mori
Contact List
AHMET KATI
contact affiliationUniversity of Health Sciences Turkey
contact emailahmet.kati@sbu.edu.tr
lab head
AHMET KATI
contact affiliationUniversity of Health Sciences
contact emailahmet.kati@sbu.edu.tr
dataset submitter
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Dataset FTP location
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